Thermo Fisher Scientific CD366 (TIM3) Monoclonal Antibody (F38-2E2), PE-eFluor 610, eBioscience

Thermo Fisher Scientific CD366 (TIM3) Monoclonal Antibody (F38-2E2), PE-eFluor 610

Applications

• Western Blot (WB): Not tested
• Flow Cytometry (Flow): 5 µL (0.25 µg)/test
  View 5 publications

Product Specifications

Available Formats

• Alexa Fluor 532
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Brilliant UV 805
• Brilliant Violet 605, 650, 786
• FITC
• Functional Grade
• PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436, 600, 645, 702, 780
• Request custom conjugation

Product Specific Information

This F38-2E2 monoclonal antibody reacts with human CD366 (TIM3, HAVCR2). CD366 is a cell surface receptor expressed on activated CD4+ T cell subsets (Th1, Th17, Treg), CD8+ T cells, monocytes, dendritic cells, and mast cells. Galectin-9 is its known ligand, and CD366 plays a role in negative regulation of CD4+ T cells, tolerance, autoimmune disease, and HIV infection.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Pre-diluted and tested by flow cytometry on human peripheral blood cells
  • Recommended: 5 µL (0.25 µg)/test (100 µL final volume)
  • Cell number: 10⁵–10⁸ cells/test (empirical)

Fluorochrome Characteristics:

• Excitation: 488–561 nm
• Emission: 607 nm
• Recommended filter: 610/20 band pass (PE-Texas Red equivalent)
• Light sensitivity: Protect from light (photo-induced oxidation)
• Fixation: Samples can be stored in IC Fixation Buffer (Product #00-8222-49) or 1-step Fix/Lyse Solution (Product #00-5333-57) up to 3 days at 4°C in dark.

Target Information

TIM3 (HAVCR2) is a 281 amino acid, type-1 Th1-specific cell surface glycoprotein expressed on terminally differentiated CD4+Th1 and CD8+Tc1 cells. It contains:

• IgV-like domain
• Mucin-like extracellular domain
• Tyrosine phosphorylation motif in cytoplasmic region

TIM3 is involved in macrophage activation, autoimmune disease induction, and immune tolerance regulation. It is linked to experimental autoimmune encephalomyelitis (EAE) and Th1-mediated immune responses.

For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.

제품 이미지