Thermo Fisher Scientific CD279 (PD-1) Monoclonal Antibody (eBioJ105 (J105)), PE-eFluor 610, eBioscience

Applications

• Flow Cytometry (Flow): 5 µL (0.5 µg)/test
  View 18 publications

Product Specifications

Additional Formats

• Unconjugated
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 615 / 661 / 737 / 805
• Brilliant Violet 421 / 480 / 650 / 711 / 786
• PE / PE-Cyanine5 / PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436 / 600 / 645 / 702 / 780
• Request custom conjugation

Product Specific Information

The J105 monoclonal antibody reacts with human PD-1 (programmed death-1), a 55 kDa member of the CD28 immunoglobulin superfamily. PD-1 contains an immunoreceptor tyrosine-based inhibitory motif (ITIM) and plays a key role in peripheral tolerance and autoimmune disease. It is expressed mainly on activated T and B lymphocytes. PD-1 ligands include PD-L1 (B7-H1) and PD-L2 (B7-DC), which are expressed on normal tissues and upregulated on activated antigen-presenting cells.

Costaining experiments indicate that eBioJ105 recognizes a different epitope than MIH4 (Product #11-9969).

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated for flow cytometry of stimulated human peripheral blood cells at 5 µL (0.5 µg) per test.
A test corresponds to staining in 100 µL final volume, typically 10⁵–10⁸ cells/test.

Fluorophore Information:
PE-eFluor® 610 is excited by 488–561 nm lasers and emits at 607 nm. Use a 610/20 band pass filter (PE-Texas Red® equivalent). Ensure instrument compatibility.

Light Sensitivity:
Tandem dye is sensitive to photo-induced oxidation. Protect vial and samples from light.

Fixation:
Samples can be stored up to 3 days at 4°C in the dark using IC Fixation Buffer (Product #00-8222) or 1-step Fix/Lyse Solution (Product #00-5333) with minimal effect on brightness.

Excitation / Emission: 488–561 nm / 607 nm
Laser Compatibility: Blue, Green, Yellow-Green
Filtration: 0.2 µm post-manufacturing filtered

Target Information

T-cell activation is regulated by inhibitory receptors such as PD-1 (PDCD-1), which binds ligands PD-L1 and PD-L2 on antigen-presenting cells. Upon ligand binding, PD-1 transmits inhibitory signals to limit immune activation in the absence of danger signals. PD-1 deficiency results in hyperactivated immune systems and autoimmune phenotypes. Despite a predicted molecular weight of 55 kDa, PD-1 migrates at higher molecular weight in SDS-PAGE.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

제품 이미지