Thermo Fisher Scientific CXCL1 Monoclonal Antibody (48415)

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

-

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Flow Cytometry (Flow)

-

View 1 publication 1 publication

ELISA (ELISA)

0.1-0.4 µg/mL (Detection), 2-8 µg/mL (Capture)

-

Neutralization (Neu)

0.05-0.25 µg/mL

-

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a

Class

Monoclonal

Type

Antibody

Clone

48415

Immunogen

E. coli-derived recombinant mouse CXCL1/GRO alpha/KC/CINC-1 Arg20-Lys96 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:CXCL1 (GRO alpha),uniProtId:P12850-1,ncbiNodeId:10090,antigenRange:20-96,antigenLength:96,antigenImageFileName:MA5-23745_CXCL1_GRO_alpha_P12850-1_Rat.svg,antigenImageFileNamePDP:MA5-23745_CXCL1_GRO_alpha_P12850-1_Rat_PDP.jpeg,sortOrder:3}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.5 mg/mL

Purification

Protein A/G

Storage buffer

PBS with 5% trehalose

Contains

No Preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2609463

Product Specific Information

This antibody shows 100% cross-reactivity with recombinant human (rh) CXCL2, less than 40% cross-reactivity with recombinant rat (rr) CINC-1, and no cross-reactivity with rrCINC-3, rhCXCL3, rhCXCL5, rhCXCL6, or recombinant mouse (rm) CXCL6.

Reconstitute at 0.5 mg/mL in sterile PBS.

Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.

Target Information

CXCL1, CXCL2, and CXCL3, also known respectively as GRO alpha, GRO beta (MIP-2 alpha) and GRO gamma (MIP-2 beta), are members of the CXC subfamily of chemokines. Mature CXCL1/2/3 proteins bind with high affinity to the IL-8 receptor type B and are potent neutrophil attractants and activators. In vitro, the processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) show a 30-fold higher chemotactic activity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.