Thermo Fisher Scientific IL-17 A/F Monoclonal Antibody (50104)

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

-

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

-

View 1 publication 1 publication

Flow Cytometry (Flow)

-

View 2 publications 2 publications

Neutralization (Neu)

0.05-0.15 µg/mL

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Human, Mouse

Host/Isotype

Rat / IgG2a

Class

Monoclonal

Type

Antibody

Clone

50104

Immunogen

E. coli-derived recombinant mouse IL-17 Thr22-Ala158 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:IL-17AF,uniProtId:Q62386-1,ncbiNodeId:10090,antigenRange:22-158,antigenLength:158,antigenImageFileName:MA5-23748_IL-17AF_Q62386-1_Rat.svg,antigenImageFileNamePDP:MA5-23748_IL-17AF_Q62386-1_Rat_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.5 mg/mL

Purification

Protein A/G

Storage buffer

PBS with 5% trehalose

Contains

No Preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2607352

Product Specific Information

In direct ELISAs, approximately 40% reactivity with recombinant mouse (rm) IL-17A/IL-17F heterodimer is observed. No cross-reactivity with recombinant human IL-17, recombinant canine IL-17, rmIL-17B, rmIL-17C, rmIL-17D, rmIL-17E, or rmIL-17F is observed.

Reconstitute at 0.5 mg/mL in sterile PBS.

Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.

Target Information

IL-17A and IL-17F are well-characterized homodimeric cytokines secreted by T helper 17 (Th17) cells, gamma-delta T cells and several subsets of innate lymphoid cells. Somewhat less appreciated, IL-17A and IL-17F subunits can also form the heterodimer, IL-17AF. Together, these three dimers signal through the IL-17RA/IL-17RC receptor complex to mediate immune responses at mucosal interfaces and are found at lesion sites in inflammatory bowel disease, asthma, atopic dermatitis and rheumatoid arthritis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.