
Thermo Fisher Scientific ORC1 Polyclonal Antibody
ORC1 단백질을 인식하는 Rabbit Polyclonal 항체로, Human, Mouse, Rat 시료에 반응합니다. Western blot에 적합하며, 항원 친화 크로마토그래피로 정제되었습니다. 액상 형태로 1 mg/mL 농도이며, 장기 보관 시 -20°C에서 보관 권장됩니다.
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Applications
Western Blot (WB)
- Tested Dilution: 1:500–1:1,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to amino acids 364–409 of Human ORC1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2719270 |
Product Specific Information
The antibody was affinity-purified from rabbit antiserum by affinity chromatography using an epitope-specific immunogen. The purity is >95% (by SDS-PAGE).
Target Information
The origin recognition complex (ORC) is a highly conserved six-subunit protein complex essential for the initiation of DNA replication in eukaryotic cells. Studies in yeast show that ORC binds specifically to origins of replication and serves as a platform for assembly of initiation factors such as Cdc6 and Mcm proteins.
The ORC1 protein is the largest subunit of this complex. Its levels vary during the cell cycle and are regulated by ubiquitin-mediated proteolysis after initiation of DNA replication. It is selectively phosphorylated during mitosis and interacts with MYST histone acetyltransferase 2 (MyST2/HBO1), involved in transcriptional silencing.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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