Thermo Fisher Scientific PDLIM7 Monoclonal Antibody (2B4A3)
PDLIM7 단백질을 인식하는 Mouse IgG1 단클론 항체로, Western blot, IHC, ICC, Flow Cytometry, ELISA 등에 사용 가능. Human 시료에 반응하며, 단백질 발현 및 세포골격 연구에 적합. Protein G 정제, 1 mg/mL 농도, PBS buffer에 0.05% sodium azide 함유.
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Applications and Tested Dilutions
| Application | Tested Dilution | References |
|---|---|---|
| Western Blot (WB) | 1:500–1:2,000 | — |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:1,000 | — |
| Immunocytochemistry (ICC/IF) | Assay-dependent | — |
| Flow Cytometry (Flow) | 1:200–1:400 | — |
| ELISA | 1:10,000 | — |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 2B4A3 |
| Immunogen | Purified recombinant fragment of human PDLIM7 (AA: 86–280) expressed in HEK293-6e cells supernatant |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C for short term; for long term, store at -20°C, avoiding freeze/thaw cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_3090838 |
Target Information
The PDLIM7 protein belongs to a family characterized by conserved PDZ and LIM domains. LIM domains facilitate protein–protein interactions involved in gene transcription, development, and cytoskeletal organization. The LIM domains bind protein kinases, while the PDZ domain binds actin filaments. PDLIM7 contributes to the assembly of actin filament-associated complexes essential for ret/ptc2 mitogenic signaling. It likely functions as an adapter protein, localizing LIM-binding partners to actin filaments in both muscle and nonmuscle cells. Alternative splicing generates multiple transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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