Thermo Fisher Scientific Complement C5a Monoclonal Antibody (557)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:50

-

ELISA (ELISA)

1:50

-

Functional Assay (Functional)

1:50

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

557

Immunogen

BALB/c mice immunized with the human C5/C5a antigen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.1 mg/mL

Purification

Protein G

Storage buffer

PBS with 0.1% BSA

Contains

no preservative

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1073836

Product Specific Information

MA1-40160 detects C5/C5a from human samples.

MA1-40160 has been successfully used in ELISA, functional assays and Western blot applications.
ELISA: antibody was used as detection antibody in ELISA.

The MA1-40160 immunogen is C5/C5a antigen.

Endotoxin <24 EU/mg.

Positive control: Recombinant C5; Negative control: C3.

Target Information

The protein encoded by this gene is the fifth component of complement, which plays an important role in inflammatory and cell killing processes. This protein is comprised of alpha and beta polypeptide chains that are linked by a disulfide bridge. An activation peptide, C5a, which is an anaphylatoxin that possesses potent spasmogenic and chemotactic activity, is derived from the alpha polypeptide via cleavage with a convertase. The C5b macromolecular cleavage product can form a complex with the C6 complement component, and this complex is the basis for formation of the membrane attack complex, which includes additional complement components. Mutations in this gene cause complement component 5 deficiency, a disease where patients show a propensity for severe recurrent infections. Defects in this gene have also been linked to a susceptibility to liver fibrosis and to rheumatoid arthritis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.