Thermo Fisher Scientific NMDAR2B Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:100-1:2,000

View 8 publications 8 publications

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

1:500-1:5,000

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

-

View 2 publications 2 publications

ELISA (ELISA)

Assay-dependent

-

Immunoprecipitation (IP)

3 µL

-

Miscellaneous PubMed (Misc)

-

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Fusion protein containing amino acid residues 984-1104 of the C-terminal region of rat brain subunit 2B if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:NMDAR2B,uniProtId:Q00960-1,ncbiNodeId:10114,antigenRange:984-1104,antigenLength:1482,antigenImageFileName:A-6474_NMDAR2B_Q00960-1_Rabbit.svg,antigenImageFileNamePDP:A-6474_NMDAR2B_Q00960-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

IgG fraction

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2536210

Product Specific Information

This antibody is specific for the ~180 kDa 2B subunit from rat, mouse and human.

Storage and reconstitution: when stored at -20ºC or below, the lyophilized antibody should retain full activity for over one year. Reconstitute using 50 µL PBS, pH 7.4, to yield a 0.2 mg/mL solution. If storing at 0-4ºC, add sodium azide to a final concentration of 2mM. For longer storage, divide the solution into aliquots and freeze at -20ºC, avoiding freeze/thaw cycles.

Target Information

NMDAR2B is a member of the N-methyl-D-aspartate (NMDA) class of ionotropic glutamate receptors. NMDA receptors (NMDAR) have been shown to be involved in long-term potentiation, an activity-dependent increase in the efficiency of synaptic transmission thought to underlie certain kinds of memory and learning. NMDAR play a role in neuronal development and has been implicated in several disorders of the central nervous system including Alzheimer`s Disease, epilepsy and ischemic neuronal cell death. Overexpression of the NR2B subunit of the receptor has been associated with increases in learning and memory while aged, memory impaired animals have deficiencies in NR2B expression. Tyr1472 on NR2B is phosphorylated and this may lead to the increased expression of the NMDAR at the synapse that plays a role in synaptic plasticity. NMDR2B is a heterotrimer comprised of seven enetically encoded and differentially expressed subunits that include NR1, NR2 and NR3. Diseases associated with the dysfunction of NMDAR2B include epileptic encephalopathy, autosomal dominant 6-related mental retardation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.