Thermo Fisher Scientific EquiPhi29 DNA Amplification Kit, 100 Reactions

Thermo Fisher Scientific EquiPhi29 DNA Amplification Kit, 100 Reactions

The Thermo Scientific™ EquiPhi29™ DNA Amplification Kit provides all necessary components for fast, sensitive, and efficient isothermal DNA amplification, including rolling circle amplification (RCA) and multiple displacement amplification – whole genome amplification (MDA-WGA).

주요 특징

• Fast DNA amplification in less than two hours
• Sensitivity from 1 fg of input DNA
• Maximal yield of 17 µg amplified DNA
• Compatible with various sample types (purified DNA, liquid media culture, agar plate colonies)
• Suitable for downstream applications such as DNA sequencing (Sanger, NGS), restriction enzyme digestion, cell-free DNA enrichment, and protein expression
• Includes all required components for RCA or MDA-WGA reactions

구성 및 특징 설명

The kit contains EquPhi29 DNA Polymerase, a proprietary phi29 DNA polymerase mutant with strong strand displacement activity, offering high thermostability, reaction speed, and product yield with low amplification bias.
It also includes an optimized reaction buffer, exo-resistant random primer, pyrophosphatase, and dNTPs.
Pyrophosphatase addition enhances DNA amplification efficiency and simplifies sample handling.

사양

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