Thermo Fisher Scientific H3K4me1 Polyclonal Antibody
H3K4me1 단일메틸화 히스톤 H3 N-말단을 인식하는 Rabbit Polyclonal 항체. Western blot, ICC/IF, IP, ChIP 등 다양한 응용에 적합. 인간, 마우스, 랫, 영장류 반응성. 항원 친화 크로마토그래피로 정제된 고품질 연구용 항체.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | View 1 publication |
| Immunocytochemistry (ICC/IF) | 1:200 | - |
| Immunoprecipitation (IP) | 1:25 | - |
| ChIP assay (ChIP) | - | View 1 publication |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human, Mouse, Non-human primate, Rat |
| Published Species | Plant |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to the amino terminus of histone H3 in which lysine 4 is mono-methylated |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 7.3 µg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | 0.01M HEPES, pH 7.5, with 0.15M NaCl, 100 µg/mL BSA, 50% glycerol |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10984402 |
Product Specific Information
- It is not recommended to aliquot this antibody.
- This antibody is not cross-reactive with methylated histone H3 Lys9, Lys27, Lys36 or methylated histone H4 Lys20.
Target Information
Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3, along with four core histone proteins, binds to DNA forming the structure of the nucleosome.
Histones play a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability.
Post-translationally, histones are modified in various ways to either directly change the chromatin structure or allow for the binding of specific transcription factors.
The N-terminal tail of histone H3 protrudes from the nucleosome core and can undergo several post-translational modifications that influence cellular processes, including methylation, acetylation, and phosphorylation.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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