Thermo Fisher Scientific Calbindin D28K Polyclonal Antibody
Calbindin D28K 단백질을 검출하기 위한 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody. 인간, 생쥐, 랫드 등 다양한 종에 반응하며 WB, IHC, ICC 등 다중 응용 가능. 고순도 항원 친화 크로마토그래피 정제, 장기 보관 시 -20°C 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:3,000 | View 1 publication |
| Immunohistochemistry (IHC) | - | View 3 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 | - |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:100–1:1,000 | - |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein encompassing a sequence within the center region of Human Calbindin |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.51 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2792808 |
Product Specific Information
- Keep as concentrated solution.
- Predicted reactivity: Mouse (97%), Rat (98%), Pig (98%), Rabbit (97%), Chicken (80%), Bovine (100%).
- Positive Control: mouse brain, rat brain.
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Calbindin-D-28K (also termed vitamin D-dependent calcium-binding protein, or cholecalcin) is a highly conserved 28kDa calcium-binding protein with broad tissue distribution. It belongs to a family of low molecular weight calcium-binding proteins (CaBPs) such as calmodulin, S-100, parvalbumin, and troponin C. These CaBPs share homologous EF-hand type structures for Ca²⁺ binding.
Calbindin-D-28K is predominantly found in subpopulations of central and peripheral nervous system neurons, and in epithelial cells involved in Ca²⁺ transport such as distal tubular cells and cortical collecting tubules of the kidney, as well as enteric neuroendocrine cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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