Thermo Fisher Scientific PMCA1 ATPase Polyclonal Antibody
PMCA1 ATPase 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot 및 IHC에 사용 가능. Human, Mouse, Rat에 반응하며, 항원 친화 크로마토그래피로 정제됨. 4°C 단기, -20°C 장기 보관 권장.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:3,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:100–1:1,000 |
| Immunohistochemistry (Frozen) (IHC-F) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human PMCA1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2792809 |
Product Specific Information
- Keep as concentrated solution.
- Predicted reactivity: Mouse (98%), Rat (98%), Dog (100%), Pig (100%), Rabbit (98%), Chicken (95%), Bovine (98%).
- Positive Control: HeLa (unboiled), HeLa MF (unboiled).
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
The calcium pump of the plasma membrane, termed PMCA ATPase, pumps calcium from the cytosol to the extracellular space. This membrane-bound enzyme is related to other ATPases including the SERCA ATPase and the sodium/potassium pump. There are four genes encoding PMCA ATPase, and studies have revealed 20 isoforms generated by alternate splicing of the primary gene products. Gene products 1 and 4 are transcribed in most tissues, while products 2 and 3 are more tissue-specific. In the pancreas, beta cells express only the 4b isoform, whereas alpha and gamma cells express both 4a and 4b isoforms. Different splice variants exhibit different affinities for calcium and calmodulin.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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