Thermo Fisher Scientific Phospho-p27 Kip1 (Ser178) Polyclonal Antibody
p27 Kip1 단백질의 Ser178 인산화 형태를 특이적으로 인식하는 polyclonal antibody. Western blot, IHC, ICC/IF에 사용 가능. 사람, 생쥐, 랫드 반응성. 세포주기 조절 연구에 적합하며, -20°C 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:3,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human CDKN1B (Accession P46527), corresponding to amino acid residues around phosphorylated Ser178. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2818500 |
Product Specific Information
This antibody detects endogenous levels of p27 Kip1 only when phosphorylated at Ser178.
Target Information
p27 Kip1 functions as a negative regulator of G1 phase progression and is proposed to mediate TGF-β-induced G1 arrest. It is a key regulator of cell cycle progression and a potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. p27 Kip1 also forms complexes with cyclin D-CDK4, contributing to assembly, stability, and modulation of CCND1-CDK4 activation. Depending on its phosphorylation state and stoichiometry, p27 Kip1 can act as either an inhibitor or activator of cyclin D-CDK4 complexes. The protein has a molecular weight of approximately 27 kDa, and its degradation—triggered by CDK-dependent phosphorylation and subsequent ubiquitination by SCF complexes—is essential for the transition from quiescence to the proliferative state.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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