Thermo Fisher Scientific Phospho-p27 Kip1 (Thr157) Polyclonal Antibody
Phospho-p27 Kip1 (Thr157) 특이적 인식 항체로, 세포주기 조절 단백질의 인산화 상태 분석에 적합. WB, IHC, ICC/IF 등에 사용 가능. Rabbit Polyclonal 형태로 높은 특이성과 재현성을 제공. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:3,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human CDKN1B (Accession P46527), corresponding to amino acid residues around phosphorylated Thr157 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2818499 |
Product Specific Information
Antibody detects endogenous levels of p27 Kip1 only when phosphorylated at Thr157.
Target Information
p27 Kip1 functions as a negative regulator of G1 phase progression and may mediate TGFβ-induced G1 arrest. It is a key regulator of cell cycle progression and inhibits cyclin E- and cyclin A-CDK2 complexes. p27 Kip1 interacts with cyclin D-CDK4 complexes, influencing their assembly and activation depending on its phosphorylation state. The protein (~27 kDa) undergoes CDK-dependent phosphorylation and ubiquitination by SCF complexes, facilitating transition from quiescence to proliferation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
제품 이미지
(이미지 파일: PA5-99566_p27Kip1_P46527-1_Rabbit.svg, PA5-99566_p27Kip1_P46527-1_Rabbit_PDP.jpeg)
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