Thermo Fisher Scientific RhoB Polyclonal Antibody
RhoB 단백질에 특이적인 Rabbit Polyclonal 항체로, Western blot 및 ELISA에 적합합니다. Human, Mouse, Rat 시료에 반응하며, Affinity Chromatography로 정제되었습니다. 세포막 및 핵 등에서 RhoB 발현 연구에 활용됩니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| ELISA | 1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to amino acids 100–190 of human RhoB (NP_004031.1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.68 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.05% ProClin 300 |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2806301 |
Product Specific Information
- Positive Samples: Mouse brain
- Cellular Location: Cell membrane, Cleavage furrow, Late endosome membrane, Lipid-anchor, Nucleus
Immunogen sequence:
VPEVKHFCPN VPIILVANKK DLRSDEHVRT ELARMKQEPV RTDDGRAMAV RIQAYDYLEC SAKTKEGVRE VFETATRAAL QKRYGSQNGC I
Target Information
Rho-related GTP-binding protein (RhoB) mediates apoptosis in neoplastically transformed cells after DNA damage. Although not essential for development, it influences cell adhesion and growth factor signaling in transformed cells. RhoB targets PKN1 to endosomes and is involved in trafficking of the EGF receptor from late endosomes to lysosomes. It is also required for stability and nuclear trafficking of AKT1/AKT, promoting endothelial cell survival during vascular development. RhoB functions as a tumor suppressor, and its deletion can lead to tumor formation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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