Thermo Fisher Scientific RhoB Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 100-190 of human RhoB (NP_0040311) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:RhoB,uniProtId:P62745-1,ncbiNodeId:9606,antigenRange:100-190,antigenLength:196,antigenImageFileName:PA5-91839_RhoB_P62745-1_Rabbit.svg,antigenImageFileNamePDP:PA5-91839_RhoB_P62745-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.68 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.05% ProClin 300

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2806301

Product Specific Information

Positive Samples: Mouse brain; Cellular Location: Cell membrane, Cleavage furrow, Late endosome membrane, Lipid-anchor, Nucleus

Immunogen sequence: VPEVKHFCPN VPIILVANKK DLRSDEHVRT ELARMKQEPV RTDDGRAMAV RIQAYDYLEC SAKTKEGVRE VFETATRAAL QKRYGSQNGC I

Target Information

Rho-related GTP-binding protein (RhoB) mediates apoptosis in neoplastically transformed cells after DNA damage. While not essential for development, it does affect cell adhesion and growth factor signaling in transformed cells. RhoB targets PKN1 to endosomes and is involved in trafficking of the EGF receptor from late endosomes to lysosomes. Also required for stability and nuclear trafficking of AKT1/AKT which promotes endothelial cell survival during vascular development. Serves as a tumor supressor - deletion of RhoB causes tumor formation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.