Thermo Fisher Scientific GABRA2 Monoclonal Antibody (N399/19), APC
GABA-A 수용체 Alpha2 서브유닛을 인식하는 마우스 단클론 항체. Western blot, IHC, ICC/IF에 사용 가능. APC 형광단백질로 결합되어 형광 분석에 적합. 사람, 마우스, 랫트 반응성. 단백질 G 정제, 4°C 보관.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (IHC) | Assay-dependent |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host/Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | N399/19 |
| Immunogen | Fusion protein amino acids 350–385 (Cytoplasmic C-terminus) of rat GABA-A Receptor Alpha2 |
| Conjugate | APC |
| Excitation/Emission Max | 651/660 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 95.64 mM phosphate / 2.48 mM MES, pH 7.4, with 0.5 M EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2932179 |
Additional Formats
- Unconjugated (MA5-27702)
- FITC (MA5-45726)
- PE (MA5-45728)
- PerCP (MA5-45727)
- Custom conjugation available upon request
Product Specific Information
- A 1:100 dilution of MA5-45725 detects GABA-A R, Alpha2 in 20 µg of mouse brain lysate by ECL immunoblot using Goat anti-mouse IgG:HRP as secondary antibody.
- Detects approximately 55 kDa.
- Does not cross-react with GABA-A Receptor Alpha1.
- This antibody was formerly sold as clone S399-19.
Target Information
GABA is the major inhibitory neurotransmitter in the mammalian brain, acting at GABA-A receptors which are ligand-gated chloride channels. Chloride conductance of these channels can be modulated by agents such as benzodiazepines that bind to the GABA-A receptor. At least 16 distinct subunits of GABA-A receptors have been identified. Alternatively spliced transcript variants encoding the same protein have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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