Thermo Fisher Scientific Spectrin beta-4 Monoclonal Antibody (N393/2), PE
Spectrin beta-4 단백질을 인식하는 마우스 단클론 항체로, PE 형광표지되어 있습니다. Western blot, IHC, ICC/IF에 사용 가능하며 인간, 생쥐, 랫드 시료에 반응합니다. 단백질 G로 정제된 액상 형태로, 4°C에서 보관합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (IHC) | Assay-dependent |
| Immunocytochemistry (ICC/IF) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | N393/2 |
| Immunogen | Fusion protein amino acids 1621–1832 (C-terminal repeats 14–15) of human Beta4-spectrin |
| Conjugate | PE (R-Phycoerythrin) |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 95.64 mM phosphate / 2.48 mM MES, pH 7.4, with 0.5 M EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2932178 |
Available Formats
- Unconjugated (MA5-27608)
- APC (MA5-45721)
- FITC (MA5-45722)
- PerCP (MA5-45723)
- Request custom conjugation
Product Specific Information
- A 1:100 dilution of MA5-45724 was sufficient for detection of Beta 4 Spectrin in 20 µg of mouse brain lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
- Detects approximately >200 kDa.
- Does not cross-react with other Beta-spectrins.
- This antibody was formerly sold as clone S393-2.
Target Information
Spectrin is the major constituent of the cytoskeletal network underlying the erythrocyte plasma membrane and determines cell shape, arrangement of transmembrane proteins, and organization of organelles. It is expressed at very low levels in many tissues, with the strongest expression in the cerebellum, spinal cord, stomach, pituitary gland, liver, pancreas, salivary gland, kidney, bladder, and heart.
Spectrin beta-4 is a non-erythrocyte member, expressed in the brain and pancreatic islets, and localized to the nuclear matrix, cytoplasmic vesicles, and PML nuclear bodies. Beta4 spectrins are essential for membrane stability and molecular organization of the nodes of Ranvier.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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