Thermo Fisher Scientific TYW1 Polyclonal Antibody
TYW1 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot 및 Immunocytochemistry에 적합합니다. 인간 시료에 반응하며, 항원 친화 크로마토그래피로 정제되었습니다. 단기 4°C, 장기 -20°C 보관 권장.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:5,000–1:20,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein fragment corresponding to a region within amino acids 480–662 of Human TYW1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.92 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | 0.1M Tris glycine, pH 7, with 20% glycerol |
| Contains | 0.01% thimerosal |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2548151 |
Product Specific Information
- Recommended positive controls: A549, HeLa, HepG2, HCT116
- Predicted reactivity: Mouse (88%), Rat (89%), Zebrafish (84%), Xenopus laevis (87%), Bovine (93%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
TYW1 (also known as TYW1A, RSAFD1, or YPL207W) is a 732 amino acid protein containing one flavodoxin-like domain that participates in the wybutosine-tRNA (Phe) biosynthesis pathway.
Wybutosine (yW) is a hypermodified guanosine at the 3-prime position adjacent to the anticodon of phenylalanine tRNA that stabilizes codon-anticodon interactions during decoding on the ribosome.
TYW1 is involved in a multistep enzymatic reaction that stabilizes codon-anticodon base-pairing during ribosomal decoding, ensuring accurate translation.
It binds to one 4Fe-4S cluster and is located on human chromosome 7.
Defects in genes on chromosome 7 have been linked to Osteogenesis imperfecta, Williams-Beuren syndrome, Lissencephaly, Citrullinemia, and Shwachman-Diamond syndrome, suggesting TYW1 may play a role in these conditions.
⚠ WARNING: This product can expose you to chemicals including mercury, known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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