
Thermo Fisher Scientific Goat anti-Human Kappa Light Chain Secondary Antibody, HRP
HRP 결합된 염소 유래 항-인간 카파 경쇄 2차 항체로, WB, IHC, ELISA에 사용 가능. 인간 IgG에 특이적이며 높은 민감도와 특이성 제공. 동결건조 형태로 안정적 저장 가능하며, 연구용으로만 사용.
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Thermo Fisher Scientific Goat anti-Human Kappa Light Chain Secondary Antibody, HRP
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:20,000 |
| Immunohistochemistry (IHC) | 1:500–1:20,000 |
| ELISA | 1:500–1:20,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Human Kappa Light Chain |
| Conjugate | HRP |
| Form | Lyophilized |
| Concentration | 2.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 1% BSA |
| Contains | 0.1% ProClin 150 |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2535630 |
| Target | Kappa light chain |
| Antibody Form | Whole Antibody |
Additional Formats
- AP conjugate
- Biotin conjugate
- FITC conjugate
- TRITC conjugate
- Custom conjugation available upon request
Product Specific Information
Each lot’s sensitivity is confirmed using ELISA, and specificity is verified by immunoelectrophoresis (IEP).
Rehydrate with 0.55 mL of deionized water and let stand 30 minutes at room temperature to dissolve (product overfilled for complete recovery). Centrifuge to remove particulates and prepare fresh working dilution daily.
Store lyophilized material at 2–8°C. For long-term storage after reconstitution, dilute with 50% glycerol and store at -20°C as liquid.
No reactivity observed to non-immunoglobulin human serum proteins, heavy chains of all human immunoglobulins, or lambda (λ) light chains.
Target Information
Anti-Human secondary antibodies are affinity-purified with well-characterized specificity for human immunoglobulins. They are useful for detection, sorting, or purification of specific targets.
Secondary antibodies enhance versatility and sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody.
Typically generated by immunizing host animals with pooled immunoglobulins from the target species, then purified and modified (e.g., immunoaffinity chromatography, fragmentation, conjugation) to produce highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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