Thermo Fisher Scientific Goat anti-Human IgG, IgM, IgA (H+L) Secondary Antibody, AP
Thermo Fisher의 염기성 포스파타제(AP) 결합 염소 유래 항-인간 IgG, IgM, IgA (H+L) 항체로, Western blot, IHC, ELISA에 적합합니다. 높은 특이성과 감도를 제공하며, 친화 크로마토그래피로 정제되었습니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:20,000 |
| Immunohistochemistry (IHC) | 1:500–1:20,000 |
| ELISA | 1:500–1:20,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Human IgG/IgA/IgM |
| Conjugate | Alkaline Phosphatase (AP) |
| Form | Liquid |
| Concentration | 2.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 30 mM triethanolamine, 1% BSA, 5 mM MgCl₂, 0.1 mM ZnCl₂ |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C (short term). For long-term storage, store at -20°C. Avoid freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2535627 |
| Target | IgG/IgA/IgM |
| Antibody Form | Whole Antibody |
Additional Formats
- [Biotin Conjugate]
- [FITC Conjugate]
- [HRP Conjugate]
- [TRITC Conjugate]
- [Request Custom Conjugation]
Product Specific Information
Each lot of this antibody is validated for sensitivity using ELISA and for specificity using immunoelectrophoresis (IEP).
Target Information
Anti-human secondary antibodies are affinity-purified and exhibit well-characterized specificity for human immunoglobulins. They are widely used for detection, sorting, or purification of human immunoglobulins.
Secondary antibodies enhance versatility by allowing use with various detection systems such as HRP, AP, or fluorescence. They also improve sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody.
These antibodies are typically generated by immunizing a host animal with pooled immunoglobulins from the target species and can be further purified or modified (e.g., immunoaffinity chromatography, antibody fragmentation, label conjugation) for high specificity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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