Thermo Fisher Scientific IQGAP1 Monoclonal Antibody (OTI5F9), TrueMAB
IQGAP1 단백질을 인식하는 Mouse monoclonal antibody로 Western blot, IHC, Flow cytometry에 사용 가능. Human, Mouse, Rat에 반응하며, 동결건조 형태로 제공. 연구용으로 세포 형태 및 이동성 조절 관련 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 1:500–1:2,000 | |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:5,000 | |
| Flow Cytometry (Flow) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI5F9 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 464–609 of human IQGAP1 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20 °C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to reach a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL (Product #89882).
Target Information
The IQGAP1 gene encodes a member of the IQGAP family. The protein contains four IQ domains, one calponin homology domain, one Ras-GAP domain, and one WW domain. It interacts with cytoskeletal components, cell adhesion molecules, and several signaling molecules to regulate cell morphology and motility. Expression is upregulated by gene amplification in some gastric cancer cell lines.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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