Thermo Fisher Scientific PCMT1 Monoclonal Antibody (OTI2E7), TrueMAB

Thermo Fisher Scientific PCMT1 Monoclonal Antibody (OTI2E7), TrueMAB

Applications

• Western Blot (WB)

Tested Dilution: 1:500–1:2,000

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).

Target Information

Protein carboxyl methyltransferases are classified into three types based on methyl-acceptor substrate specificity.
Type II enzyme catalyzes the transfer of a methyl group from S-adenosyl-L-methionine to free carboxyl groups of D-aspartyl and L-isoaspartyl residues. These residues arise from spontaneous deamidation, isomerization, and racemization of normal L-aspartyl and L-asparaginyl residues, representing covalent damage sites in aging proteins.
PCMT1 (EC 2.1.1.77) acts as a protein repair enzyme that converts abnormal residues back to the normal L-aspartyl form.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.