Thermo Fisher Scientific WIPI2 Polyclonal Antibody
WIPI2 단백질을 표적하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody. Western blot, IHC, ICC/IF에 사용 가능하며 Human, Rat 반응성 확인됨. 고순도 항원 친화 크로마토그래피 정제, 장기 보관 시 -20°C 권장.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:3,000 | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 | - |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Rat |
| Published Species | Not Applicable |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of Human WIPI2. The exact sequence is proprietary. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.57 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2736796 |
Product Specific Information
- Positive Control: K562, THP-1, Jurkat
- Predicted Reactivity: Mouse (100%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
WD repeat proteins play a role in many essential biological functions, regulating the assembly of multiprotein complexes by presenting a beta-propeller platform for simultaneous and reversible protein-protein interactions.
WIPI2 (also known as ATG18B or ATG21) is a human homolog to yeast ATG18 and contains three WD repeats forming a 7-bladed propeller structure with a conserved motif facilitating interaction with other proteins.
It is recruited to early autophagosomal structures along with Atg16L and ULK1 and is required for the formation of LC3-positive autophagosomes.
Along with the highly related WIPI1, WIPI2 is found at the plasma membrane in addition to autophagosomal membranes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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