Thermo Fisher Scientific Gemin 5 Polyclonal Antibody
Gemin 5 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody. Western blot, IHC, ICC/IF, IP 등 다양한 응용에 적합. 항원 친화 크로마토그래피로 정제된 고순도 항체로, 인간 및 마우스 반응성. 연구용으로만 사용.
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Thermo Fisher Scientific Gemin 5 Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:3,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 |
| Immunoprecipitation (IP) | 1:100–1:500 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human Gemin 5 (exact sequence proprietary) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.43 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2735832 |
Product Specific Information
- Positive Control: HeLa, HepG2
- Store product as a concentrated solution.
- Centrifuge briefly prior to opening the vial.
Target Information
The SMN complex plays a catalytic role in assembling small nuclear ribonucleoproteins (snRNPs), essential components of the spliceosome. This complex is critical for pre-mRNA splicing. Most spliceosomal snRNPs contain a common set of Sm proteins (SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF, SNRPG) that form a heptameric ring on the Sm site of snRNA. In the cytosol, Sm proteins are held in an inactive 6S pICln-Sm complex by chaperone CLNS1A. The SMN complex dissociates CLNS1A and facilitates Sm protein transfer to form core snRNPs, which are then transported to the nucleus. GEMIN5 acts as the snRNA-binding protein of the SMN complex.
Usage Note
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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