Thermo Fisher Scientific CD90.2 (Thy-1.2) Monoclonal Antibody (53-2.1), NovaFluor Blue 510, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 0.4 µg/test

Product Specifications

Available Formats

• Unconjugated
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 615
• eFluor 450
• eFluor 506
• FITC
• NovaFluor Blue 555
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Blue 760
• NovaFluor Red 710
• NovaFluor Yellow 570
• NovaFluor Yellow 660
• PE
• PE-Cyanine5
• PE-Cyanine7
• PE-eFluor 610
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Super Bright 702
• Request custom conjugation available

Product Specific Information

The 53-2.1 monoclonal antibody reacts with mouse CD90.2 (Thy-1.2), a GPI-linked membrane molecule expressed by mouse thymocytes, mature T cells, and neurons in CD90.2-expressing strains such as BALB/c, CBA, C3H, C57BL/6, C58, and SJL. Cells from CD90.1-expressing strains (e.g., PL, AKR) do not stain with 53-2.1. CD90 is involved in regulation of adhesion and signal transduction by T cells.

Each product contains:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Tested on mouse splenocytes at ≤0.4 µg/test (100 µL final volume). Optimal titration recommended.

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability testing with NovaFluor dyes.
• CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) is required for optimal staining.
  • Add 5 µL per sample before combining with cells.
  • For single-color controls: add 5 µL per 100 µL cell sample (10³–10⁸ cells).

NovaFluor conjugates utilize Phiton™ technology, providing engineered fluorescent signatures optimized for minimal spillover and spread.

Fluorescence Parameters:

• Excitation: 496 nm
• Emission: 511 nm
• Laser: 488 nm (Blue)

Target Information

CD90.2 (Thy-1) is a GPI-linked glycoprotein of the immunoglobulin superfamily. It is expressed in murine T cells, thymocytes, neural cells, Kupffer’s cells, and fibroblasts. It plays roles in cell-cell and cell-ligand interactions during neural development and other biological processes. In mice, CD90.2 is expressed on all thymocytes and peripheral T lymphocytes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

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