Thermo Fisher Scientific CD45.2 Monoclonal Antibody (104), NovaFluor Blue 660-120S, eBioscience

Thermo Fisher Scientific CD45.2 Monoclonal Antibody (Clone 104), NovaFluor Blue 660-120S

Applications

• Flow Cytometry (Flow)

Tested Dilution: 0.8 µg/test

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 532
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 395, 496, 563, 615, 661, 737, 805
• Brilliant Violet 421, 605, 650, 711, 786
• eFluor 450, 506
• FITC
• NovaFluor Blue 510, 555, 585, 610-70S
• PE, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 436, 600, 645, 780
• Request custom conjugation available

Product Specific Information

Description:
The 104 monoclonal antibody reacts with mouse CD45 (leukocyte common antigen, LCA) in CD45.2-expressing strains such as C57BL/6, BALB/c, DBA/1, DBA/2, C3H/He, CBA, 129, A, and AKR. CD45.2 is expressed by all leukocytes in these strains.

Each product contains:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Tested on mouse splenocytes. Recommended ≤0.5 µg/test (100 µL final volume). Optimize by titration.

Note:
NovaFluor dyes are incompatible with DNA intercalating viability dyes (PI, 7-AAD, DAPI). Use Invitrogen LIVE/DEAD Fixable Dead Cell stains instead.

CellBlox Plus Blocking Buffer:
Required for optimal staining with NovaFluor conjugates. Add 5 µL per sample to antibody cocktails or single-color controls (10³–10⁸ cells/sample).

Technology:
NovaFluor conjugates utilize Phiton™ technology with engineered fluorescent signatures minimizing spillover and spread.

Excitation: 509 nm
Emission: 665 nm
Laser: 488 nm (Blue)

Target Information

CD45.2 (LCA, leukocyte common antigen):
A receptor-type protein tyrosine phosphatase expressed on all nucleated hematopoietic cells (~10% of lymphocyte surface proteins). Plays critical roles in lymphocyte development, antigen signaling, and Src-family kinase regulation. Exists as multiple isoforms via alternative splicing, differing in extracellular domains. Also involved in integrin-mediated signaling and apoptosis regulation.

For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.

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