Thermo Fisher Scientific PARP1 (cleaved Asp214, Asp215) Polyclonal Antibody
Cleaved PARP1(Asp214, Asp215)을 인식하는 Rabbit Polyclonal 항체로, apoptosis 검출에 유용. WB, IHC, ICC 등 다양한 응용 가능. Jurkat 및 HeLa 세포에서 검증됨. 항원 친화 크로마토그래피로 정제된 액상 형태.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | View 12 publications |
| Immunohistochemistry (IHC) | - | View 3 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent | View 1 publication |
| Immunocytochemistry (ICC/IF) | - | View 1 publication |
| Flow Cytometry (Flow) | - | View 8 publications |
| Miscellaneous PubMed (Misc) | - | View 2 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Bovine, Human, Mouse, Rat |
| Published Species | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Chemically synthesized peptide corresponding to the N-terminus of cleavage site (214/215) of human PARP |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification | Antigen affinity chromatography |
| Storage Buffer | Dulbecco’s PBS (pH 7.3) with 1 mg/mL BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2533726 |
Product Specific Information
This antibody specifically recognizes the 85 kDa fragment of cleaved PARP and can be used as a marker for detecting apoptotic cells. It has been validated in western blotting and immunostaining applications. Positive controls include Jurkat or HeLa cells treated with staurosporine or etoposide (25 µM for 3 hours).
Target Information
Poly ADP-Ribose Polymerase (PARP) utilizes NAD (oxidized form) as a substrate to transfer ADP-ribose to nuclear protein acceptors. Proteolytic cleavage of PARP to its stable 85 kDa fragment is an early marker of apoptosis, mediated by the caspase CPP32 protein. Cleavage occurs between Asp216 and Gly217, a conserved site across species.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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