Thermo Fisher Scientific His tag Recombinant Rabbit Monoclonal Antibody (OR-5B12), TrueRAB
His tag 단백질 검출용 재조합 토끼 모노클로날 항체로, Western blot에 최적화되어 있습니다. HEK293 세포에서 발현된 TrueRAB 형식이며, 고순도 액상 형태로 제공됩니다. 6x His 태그 단백질의 특이적 검출 및 면역침강에 적합합니다.
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Applications
- Western Blot (WB): Tested dilution 1:500
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | OR-5B12 |
| Immunogen | Synthetic peptide |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5–1 mg/mL |
| Storage Buffer | PBS with 1% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | −20 °C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
Product Specific Information
Synthetic peptide sequence is HHHHHH.
Target Information
The 6x His tag is a synthetic oligopeptide consisting of six consecutive histidine residues (HHHHHH).
His tag is commonly expressed at either the N- or C-terminal regions of recombinant proteins to enable isolation or purification using immobilized metal affinity chromatography (IMAC).
Epitope tagging allows detection of recombinant proteins for which no specific antibody exists by fusing a known epitope sequence.
His-tagged proteins are purified efficiently due to strong and selective chelation between histidine clusters and Ni²⁺ ions on Ni²⁺-NTA adsorbents.
His tag-specific antibodies facilitate detection and co-immunoprecipitation of tagged proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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