Thermo Fisher Scientific CD278 (ICOS) Monoclonal Antibody (7E.17G9), FITC, eBioscience
마우스 ICOS(CD278) 인식하는 7E.17G9 클론 단일클론 항체로, FITC 형광 표지되어 있음. Flow cytometry 분석에 최적화되어 있으며, 0.25 µg/test 농도로 사용 가능. 488 nm/520 nm 파장에서 청색 레이저 검출. 면역 반응 및 세포 활성 연구용.
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Thermo Fisher Scientific CD278 (ICOS) Monoclonal Antibody (7E.17G9), FITC, eBioscience™
Applications
- Flow Cytometry (Flow)
Tested Dilution: 0.25 µg/test
Publications: View 14 publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Rat / IgG2b, kappa |
| Recommended Isotype Control | Rat IgG2b kappa Isotype Control (eB149/10H5), FITC, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 7E.17G9 |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11218705 |
Additional Formats
- Unconjugated
- APC
- Biotin
- Functional Grade
- PE
- PE-Cyanine5
- PE-Cyanine7
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 645
- Request custom conjugation
Product Specific Information
The 7E.17G9 monoclonal antibody reacts with mouse ICOS (Inducible COStimulatory molecule), a T cell-specific molecule and a third member of the CD28/CTLA-4 family.
ICOS is a 47–57 kDa homodimer expressed on activated T cells and plays a crucial role in T cell activation, proliferation, and humoral immune response.
It binds to its ligand B7h/B7RP-1 on activated APCs including B cells and may play a protective role in inflammatory autoimmune diseases.
ICOS is also involved in Th2 cell development.
Applications Reported: Flow cytometric analysis.
Applications Tested: Flow cytometric analysis of Con A-stimulated mouse splenocytes.
Recommended usage ≤ 0.25 µg per test (100 µL final volume).
Cell number: 10⁵–10⁸ cells/test (empirical determination required).
Careful titration recommended for optimal performance.
Excitation: 488 nm
Emission: 520 nm
Laser: Blue Laser
Filtration: 0.2 µm post-manufacturing filtered
Target Information
ICOS (CD278) encodes a protein belonging to the CD28 and CTLA-4 receptor family.
Functions as a homodimer and plays a key role in cell-cell signaling, immune responses, and regulation of cell proliferation.
Associated with disorders such as Immunodeficiency, Common Variable, 1 and Common Variable Immunodeficiency.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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