Thermo Fisher Scientific Cytokeratin 8 Monoclonal Antibody (LP3K), FITC, eBioscience

Thermo Fisher Scientific Cytokeratin 8 Monoclonal Antibody (LP3K), FITC, eBioscience

Applications

• Immunohistochemistry (IHC): Assay-dependent
• Immunocytochemistry (ICC/IF): Assay-dependent

Product Specifications

Product Specific Information

This LP3K monoclonal antibody reacts with human cytokeratin 8 (K8), a 55-kDa intermediate filament protein expressed in epithelial and carcinoma cells. Cytokeratin 8, a type II keratin, contributes to cellular integrity and stability and is involved in apoptosis protection and cell cycle regulation. It is often co-expressed with cytokeratin 18, forming heterodimers. In tumor cells, cytokeratin 8 can localize to the plasma membrane and is phosphorylated on serine 73 in dividing cells.

• Applications Reported: Immunofluorescent microscopy
• Applications Tested: Immunofluorescent staining of methanol-fixed MCF-7 cells (≤10 µg/mL recommended; titration advised)
• Excitation: 488 nm
• Emission: 520 nm
• Laser: Blue Laser
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

Cytokeratin 8 belongs to the type II keratin family located on chromosome 12. It forms intermediate-sized filaments with type I keratin (keratin 18) in epithelial cells, maintaining structural integrity and participating in signal transduction and differentiation. Mutations in this gene are associated with cryptogenic cirrhosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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