
Thermo Fisher Scientific Cytokeratin 8 Monoclonal Antibody (LP3K), FITC, eBioscience
인간 Cytokeratin 8을 인식하는 LP3K 단클론 항체로, FITC 형광 표지되어 있습니다. 면역형광 및 면역조직화학 분석에 적합하며, 498/517 nm의 형광 특성을 가집니다. 세포 구조 및 분화 연구용으로 사용됩니다.
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Thermo Fisher Scientific Cytokeratin 8 Monoclonal Antibody (LP3K), FITC, eBioscience
Applications
- Immunohistochemistry (IHC): Assay-dependent
- Immunocytochemistry (ICC/IF): Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | LP3K |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10548964 |
Product Specific Information
This LP3K monoclonal antibody reacts with human cytokeratin 8 (K8), a 55-kDa intermediate filament protein expressed in epithelial and carcinoma cells. Cytokeratin 8, a type II keratin, contributes to cellular integrity and stability and is involved in apoptosis protection and cell cycle regulation. It is often co-expressed with cytokeratin 18, forming heterodimers. In tumor cells, cytokeratin 8 can localize to the plasma membrane and is phosphorylated on serine 73 in dividing cells.
- Applications Reported: Immunofluorescent microscopy
- Applications Tested: Immunofluorescent staining of methanol-fixed MCF-7 cells (≤10 µg/mL recommended; titration advised)
- Excitation: 488 nm
- Emission: 520 nm
- Laser: Blue Laser
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
Cytokeratin 8 belongs to the type II keratin family located on chromosome 12. It forms intermediate-sized filaments with type I keratin (keratin 18) in epithelial cells, maintaining structural integrity and participating in signal transduction and differentiation. Mutations in this gene are associated with cryptogenic cirrhosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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