Thermo Fisher Scientific ROR gamma (t) Monoclonal Antibody (AFKJS-9), eBioscience

Applications

Tested Dilution

Publications

Western Blot (WB)

5 µg/mL

View 21 publications 21 publications

Immunohistochemistry (IHC)

-

View 12 publications 12 publications

Immunohistochemistry (Paraffin) (IHC (P))

-

View 2 publications 2 publications

Immunohistochemistry (Frozen) (IHC (F))

-

View 4 publications 4 publications

Immunocytochemistry (ICC/IF)

-

View 2 publications 2 publications

Flow Cytometry (Flow)

-

View 49 publications 49 publications

Immunoprecipitation (IP)

Assay-Dependent

View 1 publication 1 publication

ChIP assay (ChIP)

-

View 6 publications 6 publications

in situ PLA (PLA)

-

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

-

View 1 publication 1 publication

Mass Spectrometry (MS)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rhesus monkey

Published species

Human, Mouse, Pig

Host/Isotype

Rat / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

AFKJS-9

Conjugate

Unconjugated Unconjugated Unconjugated

View additional formats

• APC
• PE
• PE-eFluor 610
• PerCP-eFluor 710
• Request custom conjugation

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2865253

Product Specific Information

Description: The AFKJS-9 monoclonal antibody reacts with the mouse, human and rhesus monkey ROR gamma (t) protein. ROR gamma is a member of the retinoic acid-related orphan receptor (ROR) family, which also includes ROR alpha and ROR beta. ROR family proteins are ligand-dependent transcription factors that play roles in multiple physiological processes. ROR gamma is expressed in several tissues including liver, lung, muscle, heart and kidney. Furthermore, it was discovered that alternative transcription results in the expression of an isoform, ROR gamma (t), which is expressed exclusively in cells of the lymphoid compartment, namely CD4+CD8+ double-positive thymocytes, Th17 cells of the periphery and lymphoid tissue inducer (LTi) cells of lymphoid organs.

The ROR gamma (t) isoform differs from ROR gamma by three unique amino acids at its amino terminus. Therefore, the AFKJS-9 antibody will react with both the ROR gamma and ROR gamma (t) isoforms.

Applications Reported: This AFKJS-9 antibody has been reported for use in chromatin immunoprecipitation and western blotting.

Applications Tested: This AFKJS-9 antibody has been tested by western blot analysis. This can be used at less than or equal to 5 µg per mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

ROR gamma, a NR1 Thyroid Hormone-Like receptor, has been shown to affect thymopoiesis, bone metabolism, T-cell apoptosis, and lymphoid organogenesis. ROR gamma has been shown to promote thymocyte survival by activating the expression of the antiapoptotic protein Bcl-x(L). ROR gamma is also required for the development of lymph nodes and Peyer`s patches. It has been shown that ROR gamma(t), a thymus-specific isoform of ROR gamma from mouse, inhibits Fas ligand expression and cytokine secretion in immature thymocytes. ROR gamma binds as a monomer to response elements composed of a single core motif, GGTCA, preceded by a 6 bp AT-rich sequence. ROR gamma expression has been documented in mouse thymus, adipose, bone, skeletal muscle, liver, and kidney, and in human skeletal muscle. ESTs have been isolated from human tissue libraries, including cancerous head/neck, germ cell, and prostate, and normal colon, head/neck, lung, liver/spleen, and pancreas.

ROR gammat t (RORgt) is an isoform of RORC.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.