Thermo Fisher Scientific ROR gamma (t) Monoclonal Antibody (AFKJS-9), eBioscience

Applications and Tested Dilutions

Product Specifications

Additional Formats

• APC ([Cat# 17-6988-82])
• PE ([Cat# 12-6988-82])
• PE-eFluor 610 ([Cat# 61-6988-82])
• PerCP-eFluor 710 ([Cat# 46-6988-82])
• Custom conjugation available upon request

Product Specific Information

The AFKJS-9 monoclonal antibody reacts with mouse, human, and rhesus monkey ROR gamma (t) protein. ROR gamma belongs to the retinoic acid-related orphan receptor (ROR) family, including ROR alpha and ROR beta. These ligand-dependent transcription factors regulate multiple physiological processes and are expressed in tissues such as liver, lung, muscle, heart, and kidney.

The ROR gamma (t) isoform is expressed exclusively in lymphoid cells, including CD4+CD8+ double-positive thymocytes, Th17 cells, and lymphoid tissue inducer (LTi) cells. It differs from ROR gamma by three amino acids at its N-terminus, allowing AFKJS-9 to recognize both isoforms.

Applications Reported: Chromatin immunoprecipitation and western blotting.
Applications Tested: Western blot analysis (≤ 5 µg/mL recommended; titrate for optimal performance).
Purity: >90% (SDS-PAGE).
Aggregation: <10% (HPLC).
Filtration: 0.2 µm post-manufacturing filtered.

Target Information

ROR gamma (NR1 Thyroid Hormone-Like receptor) influences thymopoiesis, bone metabolism, T-cell apoptosis, and lymphoid organogenesis. It promotes thymocyte survival via Bcl-x(L) expression and is essential for lymph node and Peyer’s patch development. ROR gamma(t), a thymus-specific isoform, modulates Fas ligand expression and cytokine secretion in immature thymocytes.

ROR gamma binds monomerically to response elements containing a GGTCA motif preceded by a 6 bp AT-rich sequence. Expression is observed in thymus, adipose, bone, skeletal muscle, liver, and kidney (mouse), and skeletal muscle (human). ESTs have been isolated from various human tissues, including normal and cancerous types.

Note: ROR gamma(t) (RORgt) is an isoform of RORC.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.