Thermo Fisher Scientific ROR gamma (t) Monoclonal Antibody (AFKJS-9), eBioscience
ROR gamma(t) 단백질을 인식하는 AFKJS-9 단클론 항체로, Human, Mouse, Rhesus monkey에 반응합니다. Western blot, IHC, Flow Cytometry 등 다양한 응용에 사용 가능하며, 0.5 mg/mL 농도의 액상 형태로 제공됩니다. 연구용으로만 사용됩니다.
- 판매단위
- pk
카탈로그
4개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific ROR gamma (t) Monoclonal Antibody (AFKJS-9), eBioscience
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 5 µg/mL | 21 |
| Immunohistochemistry (IHC) | - | 12 |
| Immunohistochemistry (Paraffin) (IHC (P)) | - | 2 |
| Immunohistochemistry (Frozen) (IHC (F)) | - | 4 |
| Immunocytochemistry (ICC/IF) | - | 2 |
| Flow Cytometry (Flow) | - | 49 |
| Immunoprecipitation (IP) | Assay-Dependent | 1 |
| ChIP assay (ChIP) | - | 6 |
| in situ PLA (PLA) | - | 1 |
| Miscellaneous PubMed (Misc) | - | 1 |
| Mass Spectrometry (MS) | - | 1 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rhesus monkey |
| Published Species | Human, Mouse, Pig |
| Host / Isotype | Rat / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | AFKJS-9 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2865253 |
Additional Formats
- APC ([Cat# 17-6988-82])
- PE ([Cat# 12-6988-82])
- PE-eFluor 610 ([Cat# 61-6988-82])
- PerCP-eFluor 710 ([Cat# 46-6988-82])
- Custom conjugation available upon request
Product Specific Information
The AFKJS-9 monoclonal antibody reacts with mouse, human, and rhesus monkey ROR gamma (t) protein. ROR gamma belongs to the retinoic acid-related orphan receptor (ROR) family, including ROR alpha and ROR beta. These ligand-dependent transcription factors regulate multiple physiological processes and are expressed in tissues such as liver, lung, muscle, heart, and kidney.
The ROR gamma (t) isoform is expressed exclusively in lymphoid cells, including CD4+CD8+ double-positive thymocytes, Th17 cells, and lymphoid tissue inducer (LTi) cells. It differs from ROR gamma by three amino acids at its N-terminus, allowing AFKJS-9 to recognize both isoforms.
Applications Reported: Chromatin immunoprecipitation and western blotting.
Applications Tested: Western blot analysis (≤ 5 µg/mL recommended; titrate for optimal performance).
Purity: >90% (SDS-PAGE).
Aggregation: <10% (HPLC).
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
ROR gamma (NR1 Thyroid Hormone-Like receptor) influences thymopoiesis, bone metabolism, T-cell apoptosis, and lymphoid organogenesis. It promotes thymocyte survival via Bcl-x(L) expression and is essential for lymph node and Peyer’s patch development. ROR gamma(t), a thymus-specific isoform, modulates Fas ligand expression and cytokine secretion in immature thymocytes.
ROR gamma binds monomerically to response elements containing a GGTCA motif preceded by a 6 bp AT-rich sequence. Expression is observed in thymus, adipose, bone, skeletal muscle, liver, and kidney (mouse), and skeletal muscle (human). ESTs have been isolated from various human tissues, including normal and cancerous types.
Note: ROR gamma(t) (RORgt) is an isoform of RORC.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.