Thermo Fisher Scientific PCNA Polyclonal Antibody
Thermo Fisher Scientific의 PCNA Polyclonal Antibody는 인간 PCNA 단백질을 인식하는 토끼 다클론 항체로, WB, IHC, ICC, Flow Cytometry에 사용됩니다. 세포 증식 마커 연구에 적합하며, 2 mg/mL 농도의 액상형 제품으로 4°C 또는 -20°C에서 보관 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:10–1:50 |
| Immunocytochemistry (ICC/IF) | 1:10–1:50 |
| Flow Cytometry (Flow) | 1:10–1:50 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH conjugated synthetic peptide between amino acids 89–117 from the central region of human PCNA |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 2 mg/mL |
| Purification | Size-exclusion – Dialysis, Ammonium sulfate precipitation |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C (short term). For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2160547 |
Product Specific Information
This antibody is predicted to react with bovine, chicken, hamster, mouse, non-human primate, and rat based on sequence homology.
Target Information
PCNA (Proliferating Cell Nuclear Antigen) is essential for DNA replication and is involved in DNA excision and mismatch repair pathways. It binds to CDK inhibitor p21, endonucleases Fen1 and XPG, and DNA cytosine 5-methyltransferase (MCMT). PCNA serves as a key marker for proliferative cells and tumor proliferation status. It is localized in the nucleus, acts as a homotrimer, and functions as a cofactor of DNA polymerase delta to enhance DNA synthesis processivity. Upon DNA damage, PCNA is ubiquitinated and participates in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been identified, and pseudogenes exist on chromosome 4 and the X chromosome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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