Thermo Fisher Scientific HMOX1 Monoclonal Antibody (GT17811)
HMOX1 단백질을 인식하는 Mouse monoclonal antibody로, Western blot, ICC/IF, ELISA에 사용 가능. Human에 반응하며, 예측 반응종에는 Mouse, Rat, Dog, Pig, Bovine 포함. 단백질 A로 정제된 액상 형태로, 1 mg/mL 농도. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution | References |
|---|---|---|
| Western Blot (WB) | 1:500–1:3,000 | – |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | – |
| ELISA | Assay-dependent | – |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | GT17811 |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Heme Oxygenase 1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2787186 |
Product Specific Information
- Keep as concentrated solution.
- Predicted reactivity: Mouse (81%), Rat (80%), Dog (85%), Pig (82%), Bovine (83%).
- Positive Control: HeLa (500 µM CoCl₂ treatment for 24 hr).
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Heme oxygenase (HO) is a microsomal enzyme that catalyzes the oxidation of heme to the antioxidant molecules, biliverdin and carbon monoxide.
HO consists of two homologous isozymes, an inducible HO-1 and a constitutively expressed HO-2.
HO-1 is induced by a wide variety of stimuli including oxidative stress, inflammatory agents, transforming growth factor beta, and heat shock.
The increase in expression of HO-1 is thought to be a cellular defense mechanism against oxidative stress since elevated HO could generate more bilirubin, an antioxidant.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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