Thermo Fisher Scientific GTF2B Monoclonal Antibody (IIB8)

Applications

Tested Dilution

Publications

Western Blot (WB)

1-10 µg/mL

-

ELISA (ELISA)

1-10 µg/mL

-

Immunoprecipitation (IP)

1-10 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

IIB8

Immunogen

Recombinant human transcription factor IIB (TFIIB).

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

~1 mg/mL

Purification

purified

Storage buffer

PBS, pH 7.4

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2114364

Product Specific Information

MA1-10884 detects Transcription Factor IIB from human and mouse samples.

MA1-10884 has been successfully used in ELISA, Western blot, and immunoprecipitation applications.

The MA1-10884 immunogen is recombinant human transcription factor IIB (TFIIB).

Target Information

Ubiquitin is a 76 amino acid highly conserved eukaryotic polypeptide that selectively marks cellular proteins for proteolytic degradation by the 26S proteasome. The process of target selection, covalent attachment and shuttle to the 26S proteasome is a vital means of regulating the concentrations of key regulatory proteins in the cell by limiting their lifespans. Polyubiquitination is a common feature of this modification. Serial steps for modification include the activation of ubiquitin, an ATP-dependent formation of a thioester bond between ubiquitin and the enzyme E1, transfer by transacylation of ubiquitin from E1 to the ubiquitin conjugating enzyme E2, and covalent linkage to the target protein directly by E2 or via E3 ligase enzyme. Deubiquitination enzymes also exist to reverse the marking of protein substrates. Posttranslational tagging by Ub is involved in a multitude of cellular processes, including the cell cycle, cell growth and differentiation, embryogenesis, apoptosis, signal transduction, DNA repair, regulation of transcription and DNA replication, transmembrane transport, stress responses, the immune response, and nervous system functions.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.