Thermo Fisher Scientific Zika Virus NS1 Monoclonal Antibody (GT5212)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:10,000

-

Immunocytochemistry (ICC/IF)

1:100-1:1,000

-

ELISA (ELISA)

1:1,000-1:10,000

-

Product Specifications

Species Reactivity

Virus

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

GT5212

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of Zika virus NS1 protein (Zika virus (strain H/PF/2013). The exact sequence is proprietary.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2911488

Product Specific Information

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Information

As a member of the Flaviviridae family, ZIKV has a single positive sense RNA genome, which is initially translated as a single polyprotein which is then cleaved post-transitionally into three structural proteins (C, PrM or M, and E) and seven nonstructural (NS) proteins (NS1, NS2A, NS2B, NS3, NS4A, NS4B and NS5)(1). The glycosated NS1 forms a homodimer inside the cells and is necessary for viral replication and late in infection (2). NS1 is also secreted into the extracellular space as a hexameric lipoprotein particle, which assists the virus in immune evasion and pathogenesis by interacting with components from both innate and adaptive immune systems as well as other host factors (1). Reference 1) Song, H.; Qi, J.; Haywood, J.; Shi, Y.; Gao, G.F. (2016). Nat. Struct. Mol. Biol. 23, 456-458. 2) Suthar, M.S., Diamond, M.S. & Gale, M. Jr. (2013). Nat. Rev. Microbiol. 11, 115-128.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.