Thermo Fisher Scientific NFkBp65 Polyclonal Antibody
NFkB p65 단백질을 인식하는 Rabbit Polyclonal Antibody로, Western blot과 IHC(Paraffin)에 사용 가능. Human, Mouse, Rat 반응성. 항원 친화 크로마토그래피로 정제되었으며, 0.5 mg/mL 농도의 액상 형태. 연구용으로만 사용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–1 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 2–10 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide derived from epitope -FRTPP- with the non-phosphorylation site Thr254 of human NF-κB p65 protein (UniProt ID: Q04206-1, antigen range: 252–256) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with proprietary stabilizer |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C (short term). For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Positive control: Mouse brain
- Cellular location: Nuclear, cytoplasmic
Target Information
Nuclear factor kB p65 (NF-κB p65) is encoded by the RELA gene located on chromosome 11 in humans. Also known as RelA (v-rel avian reticuloendotheliosis viral oncogene homolog A), it belongs to the Rel family of proteins.
NF-κB p65 forms a heterodimer with p50 or p52 subunits and plays a central role in the transcription of the immunoglobulin kappa (κ) gene in mature B-lymphoid cells. Upon TNF-α binding to its receptor, IKK is phosphorylated, leading to phosphorylation and proteasomal degradation of IκB, thereby activating NF-κB signaling.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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