Norgen COVID-19/Influenza (A & B) TaqMan RT-PCR Kit

Norgen COVID-19/Influenza (A & B) TaqMan RT-PCR Detection Kit

SKU: TM67400

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Features and Benefits

• Multiplex RT-PCR system detects SARS-CoV-2 (N gene), Influenza A (M gene), and Influenza B (HA gene) in a single one-step RT-PCR reaction
• Primer & Probe Mixes include N, M, HA, and human RP assays
• Positive Control includes SARS-CoV-2 N gene, Influenza A M gene, Influenza B HA gene, and human RP internal control
• Ready-to-use 2X One-Step RT-PCR Master Mix included
• Manufactured under ISO 13485:2016 for research use only
• CE-IVD marked version pending

View High Resolution Data

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Kit Description

The kit includes 2X One-Step RT-PCR Master Mix, primer/probe mix, positive control, and negative control (nuclease-free water).
Primer/probe mix targets:

• SARS-CoV-2 (N gene)
• Influenza A (M gene)
• Influenza B (HA gene)
• Human RNase P transcript (internal control)

The positive control contains in vitro RNA transcripts for N, M, HA, and RP genes.

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Compatible PCR Instruments

• Qiagen Rotor-Gene Q
• BioRad CFX96 Touch™ Real-Time PCR Detection System
• ABI 7500
• ABI QuantStudio 5 and 7

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Supporting Data

Limit of Detection

PCR amplification of nasopharyngeal, oropharyngeal, and saliva samples spiked with SARS-CoV-2, Influenza A, and Influenza B viral particles at 8X, 80X, 800X, and 8,000X LoD.
Detection possible across all sample types with similar Ct values.

OCR Extracted Data (Figure 1):

A. Nasopharyngeal Swabs
| Virus | LoD Level | Ct Value |
|--------|------------|----------|
| Influenza A | 8,000X / 80X / 8X | (Ct bars) |
| Influenza B | 8,000X / 80X / 8X | (Ct bars) |
| SARS-CoV-2 | 8,000X / 80X / 8X | (Ct bars) |

B. Oropharyngeal Swabs
| Virus | LoD Level | Ct Value |
|--------|------------|----------|
| Influenza A | 8,000X / 80X / 8X | (Ct bars) |
| Influenza B | 8,000X / 80X / 8X | (Ct bars) |
| SARS-CoV-2 | 8,000X / 80X / 8X | (Ct bars) |

C. Saliva Samples
| Virus | LoD Level | Ct Value |
|--------|------------|----------|
| Influenza A | 8,000X / 80X / 8X | (Ct bars) |
| Influenza B | 8,000X / 80X / 8X | (Ct bars) |
| SARS-CoV-2 | 8,000X / 80X / 8X | (Ct bars) |

Legend:
■ Influenza A ■ Influenza B ■ SARS-CoV-2

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Robustness

Ct values from nasopharyngeal and saliva samples spiked with viral particles at 3X LoD.
Interfering substances did not affect Ct values.

OCR Extracted Data (Figure 2):

A. Nasopharyngeal Swabs
| Condition | Influenza A | Influenza B | SARS-CoV-2 |
|------------|--------------|--------------|-------------|
| ddH₂O | (Ct bar) | (Ct bar) | (Ct bar) |
| RNeasy | (Ct bar) | (Ct bar) | (Ct bar) |
| MagMAX | (Ct bar) | (Ct bar) | (Ct bar) |
| QIAamp | (Ct bar) | (Ct bar) | (Ct bar) |
| Chemagen | (Ct bar) | (Ct bar) | (Ct bar) |
| PureLink | (Ct bar) | (Ct bar) | (Ct bar) |
| Promega | (Ct bar) | (Ct bar) | (Ct bar) |
| Norgen | (Ct bar) | (Ct bar) | (Ct bar) |

B. Saliva Samples
| Condition | Influenza A | Influenza B | SARS-CoV-2 |
|------------|--------------|--------------|-------------|
| ddH₂O | (Ct bar) | (Ct bar) | (Ct bar) |
| Blood | (Ct bar) | (Ct bar) | (Ct bar) |
| Mucus | (Ct bar) | (Ct bar) | (Ct bar) |
| Chloraseptic | (Ct bar) | (Ct bar) | (Ct bar) |
| Toothpaste | (Ct bar) | (Ct bar) | (Ct bar) |
| Mouthwash | (Ct bar) | (Ct bar) | (Ct bar) |
| Cepacol | (Ct bar) | (Ct bar) | (Ct bar) |
| Ricola/Halls | (Ct bar) | (Ct bar) | (Ct bar) |

Legend:
Influenza A (dark blue), Influenza B (medium blue), SARS-CoV-2 (light blue)

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Freeze/Thaw Stability

Stable detection after up to 4 freeze/thaw cycles at 2X and 5X LoD.

OCR Extracted Data (Figure 3):

Legend: Cycle1–Cycle4
No significant change in Ct values across cycles.

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Storage Conditions and Product Stability

All components should be stored at -20°C upon arrival.
Avoid repeated thawing/freezing (>2x).
For intermittent use, freeze reagents in aliquots.
Stable for 6 months at -20°C without performance loss.

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Kit Components

† Contains in vitro RNA transcripts for SARS-CoV-2 N gene, Influenza M and HA genes, and RNase P RNA fragments.

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Documentation

• Protocol (500 rxns)
• Safety Data Sheet (SDS)

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Related Products

• Total Nucleic Acid Preservation Tubes
• Saliva RNA Collection and Preservation Devices
• Total RNA Purification Kit

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