Thermo Fisher Scientific p53 Polyclonal Antibody
Thermo Fisher Scientific의 p53 Polyclonal Antibody는 인간 p53 단백질을 인식하는 토끼 다클론 항체입니다. WB, IHC, ICC, IP, ChIP 등 다양한 응용에 적합하며, 항원 친화 크로마토그래피로 정제되었습니다. 암 연구 및 세포 스트레스 반응 연구에 활용됩니다.
- 카탈로그번호
- PA527822
- 판매단위
- pk
카탈로그
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:3,000 | View 2 publications |
| Immunohistochemistry (IHC) | – | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 | – |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | – |
| Immunoprecipitation (IP) | 1:100–1:500 | View 2 publications |
| ChIP assay (ChIP) | Assay-dependent | – |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Virus |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to amino acids 142–393 of Human p53 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.28 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2545298 |
Product Specific Information
- Recommended positive controls: 293T, A431, HCT116, HCT116 (24hr cisplatin 30 µM), HUVEC
- Predicted reactivity: Rat (86%), Dog (91%), Cat (90%), Pig (89%), Rabbit (92%), Sheep (86%), Rhesus Monkey (97%), Bovine (86%), Guinea pig (83%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
The tumor suppressor protein p53 is a sequence-specific transcription factor activated by cellular stress. It mediates cell cycle arrest or apoptosis in response to DNA damage or nutrient deprivation. p53 up-regulates stress response genes such as p21waf1, leading to G1 or G2/M arrest.
Structurally, p53 includes:
- N-terminal transactivation domain
- Central DNA-binding domain
- Oligomerization domain
- C-terminal regulatory domain
Phosphorylation at Ser15 is crucial for activation and stabilization, while phosphorylation at Ser392 is associated with tumor formation. p53 plays key roles in tumor suppression, aging, oxidative stress, and memory-related processes. Mutations in TP53 are linked to various cancers and hereditary syndromes such as Li-Fraumeni syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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