Thermo Fisher Scientific Aconitase 1 Polyclonal Antibody
Thermo Fisher Scientific의 Aconitase 1 폴리클로날 항체는 인간 ACO1 단백질을 검출하기 위한 Rabbit IgG 항체입니다. Western blot 및 Immunocytochemistry에 적합하며, 항원 친화 크로마토그래피로 정제되었습니다. 세포 내 철 항상성 연구에 유용합니다.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:3,000 | View 1 publication |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to a region within amino acids 83–461 of Human Aconitase 1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.78 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | 0.1M Tris Glycine, pH 7, with 20% glycerol |
| Contains | 0.01% thimerosal |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2545300 |
Product Specific Information
- Recommended positive controls: A549, HeLa, HepG2, HCT116
- Predicted reactivity: Mouse (93%), Rat (93%), Zebrafish (81%), Xenopus laevis (85%), Rabbit (94%), Chicken (85%), Rhesus Monkey (99%), Bovine (94%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Aconitase 1 (ACO1), also known as iron regulatory element binding protein 1 (IREB1), is a cytosolic protein that binds to iron-responsive elements (IREs). It plays a central role in cellular iron homeostasis and exhibits aconitase enzyme activity.
IREs are stem-loop structures found in the 5′ UTR of ferritin mRNA and the 3′ UTR of transferrin receptor mRNA. Iron-induced binding to the IRE represses ferritin mRNA translation and stabilizes transferrin receptor mRNA, thus maintaining iron balance within the cell.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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