Thermo Fisher Scientific Phospho-SMAD2/SMAD3 (Ser465, Ser467, Ser423, Ser425) Recombinant Rabbit Monoclonal Antibody (6P1T1)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:5,000

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

6P1T1

Immunogen

A synthetic phosphorylated peptide around S465 & S467 of human SMAD2 (NP_005892.1). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:SMAD2/SMAD3,uniProtId:Q15796-1,ncbiNodeId:9606,antigenRange:464-467,antigenLength:467,antigenImageFileName:MA5-51556_SMAD2SMAD3_Q15796-1_Rabbit.svg,antigenImageFileNamePDP:MA5-51556_SMAD2SMAD3_Q15796-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 0.05% BSA, 50% glycerol

Contains

0.05% ProClin 300

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_3094408

Product Specific Information

Immunogen sequence: SSMS

Target Information

Smad proteins, the mammalian homologs of the Drosophila mothers against decapentaplegic (Mad), have been implicated as downstream effectors of TGFβ/BMP signaling. Smad1 (also designated Madr1 or JV4-1) and Smad5 are effectors of BMP-2 and BMP-4 function, while Smad2 (also designated Madr2 or JV18-1) and Smad3 are involved in TGFβ and Activin-mediated growth modulation. Smad4 (also designated DPC4) has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regulate the response to Activin/TGFβ signaling by interfering with TGFβ-mediated phosphorylation of other Smad proteins.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.