Thermo Fisher Scientific Histone H3 Polyclonal Antibody
Western blot, IHC, IP에 적합한 Rabbit Polyclonal 항체. Human 및 Mouse에 반응하며, 다양한 종에 예측 반응. 항원 친화 크로마토그래피로 정제된 액상 항체로, 4°C 보관. 염색체 안정성 연구 및 히스톤 변형 연구에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution / Amount |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:1,000 |
| Immunoprecipitation (IP) | 2–10 µg/mg lysate |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 100 and the C-terminus (residue 135) of human Histone H3 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.20 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | TBS, pH 7.0–8.0, with 0.1% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- For IHC, epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections.
- Based on 100% sequence identity, this antibody is predicted to react with Mouse, Rat, D. melanogaster, Zebrafish, X. laevis, X. tropicalis, Chicken, Bovine, Rabbit, Pig, and Orangutan.
Target Information
Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3, along with four core histone proteins, binds to DNA to form the nucleosome structure. Histones play a central role in transcription regulation, DNA repair, replication, and chromosomal stability.
Post-translational modifications of histones can alter chromatin structure or facilitate transcription factor binding. The N-terminal tail of histone H3 undergoes various modifications such as methylation, acetylation, and phosphorylation, influencing key cellular processes.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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