Thermo Fisher Scientific Histone H3 Polyclonal Antibody
Rabbit polyclonal antibody targeting human Histone H3, suitable for WB, IHC(P), and IP applications. Recognizes C-terminal region (residues 100–135). Unconjugated, liquid form, stored at 4°C. Predicted cross-reactivity with multiple species.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:1,000 |
| Immunoprecipitation (IP) | 2–10 µg/mg lysate |
Product Specifications
| Property | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 100 and the C-terminus (residue 135) of human Histone H3 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.20 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- For IHC, epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections
- Based on 100% sequence identity, predicted to react with Mouse, Rat, D. melanogaster, Zebrafish, X. laevis, X. tropicalis, Chicken, Bovine, Rabbit, Pig, and Orangutan
Target Information
Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. Together with other core histones, it forms the nucleosome structure that organizes DNA. Histones are central to transcription regulation, DNA repair, replication, and chromosomal stability. The N-terminal tail of histone H3 can undergo post-translational modifications such as methylation, acetylation, and phosphorylation, influencing chromatin structure and gene expression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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