Thermo Fisher Scientific NUMB Polyclonal Antibody
Rabbit polyclonal antibody recognizing NUMB protein. Validated for WB, IHC(P), and ICC/IF applications. Reacts with human, mouse, rat, bovine, and primate samples. Supplied as liquid, 0.5 mg/mL, purified by antigen affinity chromatography. For research...
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 5–10 µg/mL |
| Immunocytochemistry (ICC/IF) | 5 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Bovine, Human, Mouse, Non-human primate, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to amino acids 12–40 of human NUMB |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2540986 |
Target Information
Protein NUMB homolog is an evolutionarily conserved protein that regulates the differentiation of neuronal progenitor cells. NUMB participates in clathrin-dependent endocytosis through binding to the AP-2 complex. It is expressed in postmitotic neurons and contributes to axonal growth. Neural cells expressing Numb isoforms with short phosphotyrosine-binding (SPTB) domains exhibit extensive neurite outgrowth, which can be inhibited by voltage-gated Ca²⁺ channel blockers or Ca²⁺ chelators.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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