Thermo Fisher Scientific DACT1 Monoclonal Antibody (OTI13H4), TrueMAB

Applications

• Western Blot (WB)
  Tested Dilution: 1:2,000

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).

Target Information

Dapper homolog 1 (DACT1), an ortholog of Xenopus Dpr, antagonizes Wnt signaling by promoting Dvl degradation via a lysosome inhibitor-sensitive and proteasome inhibitor-insensitive mechanism.
It interacts with Dvl through the DEP domain and the central/C-terminal regions of DACT1.
The human DACT1 gene is located on chromosome 14q23.1 and regulates CTNNB1/beta-catenin degradation, enhancing Wnt pathway transcriptional activation.
DACT1 is essential for normal notochord formation and is downregulated in hepatocellular carcinoma (HCC). It is expressed in human liver and various tissues.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.