Thermo Fisher Scientific APE1 Monoclonal Antibody (13B 8E5C2)
APE1 단백질을 인식하는 Mouse IgG2a 단클론 항체로, Western blot, IHC, Flow cytometry, IP 등 다양한 응용 가능. Human, Mouse, Rat 등 여러 종에 반응하며, 고순도 정제 및 PBS buffer에 보관. DNA 복구 및 산화 신호 조절 연구에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | View 2 publications |
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:1,000 | - |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:5,000 | - |
| Immunocytochemistry (ICC/IF) | 1:50–1:500 | View 1 publication |
| Flow Cytometry (Flow) | 2–5 µg/mL | - |
| Immunoprecipitation (IP) | 5 µg | - |
| ChIP assay (ChIP) | 1.5 µg / 10^6 cells | - |
| RNA Immunoprecipitation (RIP) | Assay-dependent | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Dog, Human, Mouse, Non-human primate, Rat |
| Published Species | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 13B 8E5C2 |
| Immunogen | Full-length recombinant human APE protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Purified |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_325426 |
Product Specific Information
MA1-440 detects apurinic/apyrimidinic endonuclease (APE/ref-1) from human, non-human primate, canine, mouse, and rat samples.
It has been successfully used in Western blot, immunohistochemical, flow cytometry, and immunoprecipitation procedures.
By Western blot, this antibody detects a 36 kDa protein representing APE/ref-1 in HeLa cell lysate.
Immunohistochemical staining of APE/ref-1 in various normal and cancerous human tissues (ovaries, cervix, prostate, germ cell tumors, osteosarcomas, rhabdosarcomas) shows nuclear staining patterns.
The immunogen is full-length recombinant human APE protein.
Target Information
Mammalian apurinic/apyrimidinic endonuclease (APE/ref-1) is a multifunctional enzyme involved in DNA repair and redox regulation of transcription factors such as Fos, Jun, NF-κB, Myb, HIF-1α, CREB, Pax, and p53.
APE repairs abasic sites in DNA and is implicated in oxidative signaling, cell cycle control, and apoptosis.
It is differentially expressed across tissues and development stages, with nuclear localization and phosphorylation regulation via casein kinase II.
Expression levels of APE increase upon reoxygenation of hypoxic cells and in certain malignant tissues, while decreases are associated with apoptosis induction.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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