Thermo Fisher Scientific MYPT1 Polyclonal Antibody
Rabbit polyclonal antibody targeting MYPT1 for WB and IP applications. Recognizes human MYPT1 (aa 800–850) and predicted to cross-react with mouse and rat. Supplied as liquid, 0.2 mg/mL, stored at 4°C. For research use only.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunoprecipitation (IP) | 2–5 µg/mg lysate |
Product Specifications
| Property | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residues 800 and 850 of human Myosin Phosphatase Target Subunit 1 (Protein Phosphatase 1, Regulatory Subunit 12A) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.20 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Based on 100% sequence identity, this antibody is predicted to react with Mouse and Rat
Target Information
Myosin phosphatase target subunit 1 (MYPT1), also known as the myosin-binding subunit of myosin phosphatase, is a regulatory subunit that controls the interaction between actin and myosin downstream of the guanosine triphosphatase Rho.
RhoA, in its GTP-bound active form, interacts with MYPT1 to regulate phosphorylation of the myosin light chain (MLC), influencing smooth muscle contraction and actin-myosin interactions in nonmuscle cells.
Rho-associated kinase (Rho-kinase), activated by GTP-RhoA, phosphorylates MYPT1, inactivating myosin phosphatase. Overexpression of RhoA or activated RhoA increases phosphorylation of MYPT1 and MLC, indicating Rho-mediated inhibition of myosin phosphatase.
Multiple transcript variants encoding different isoforms have been identified for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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