Thermo Fisher Scientific Rat anti-Mouse IgG1 Secondary Antibody, PE-Cyanine7, eBioscience
Flow cytometry용 Rat anti-Mouse IgG1 2차 항체로, PE-Cyanine7으로 결합되어 높은 형광 신호를 제공합니다. Mouse IgG1에 특이적이며, 다른 아이소타입 또는 Rat 항체와 교차 반응하지 않습니다. 0.5 µg/test 농도로 사용 권장, 4°C 암소 보관.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Flow Cytometry (Flow) | 0.5 µg/test |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Rat / IgG |
| Class | Monoclonal |
| Type | Secondary Antibody |
| Clone | M1-14D12 |
| Conjugate | PE-Cyanine7 |
| Excitation / Emission Max | 569 / 780 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11150243 |
| Target | IgG1 |
| Antibody Form | Whole Antibody |
Additional Conjugate Formats
- APC
- Biotin
- eFluor 450
- eFluor 570
- eFluor 615
- eFluor 660
- FITC
- HRP
- PE
- PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 600
- Super Bright 780
- Request custom conjugation available
Product Specific Information
The monoclonal antibody M1-14D12 recognizes mouse IgG1 antibodies and is suitable as a secondary reagent in flow cytometry and microscopy. It does not recognize other mouse isotypes and shows no cross-reactivity with rat IgG1 or other rat isotypes.
Applications Reported: Flow cytometric analysis.
Applications Tested: Tested by flow cytometry of cells stained with a mouse IgG1 primary antibody. Recommended ≤0.5 µg/test (100 µL final volume, 10⁵–10⁸ cells/test). Optimize titration for best results.
Light Sensitivity: The tandem dye is sensitive to photo-induced oxidation. Protect vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product #00-8222-49) or 1-step Fix/Lyse Solution (Product #00-5333-54) for up to 3 days at 4°C in the dark with minimal impact on brightness and FRET efficiency. Clone-specific performance should be verified empirically.
Fluorescence Properties:
- Excitation: 488–561 nm
- Emission: 775 nm
- Compatible Lasers: Blue, Green, Yellow-Green
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Anti-Mouse secondary antibodies are affinity-purified with well-characterized specificity for mouse immunoglobulins. They are useful for detection, sorting, or purification of target antibodies. Secondary antibodies enhance versatility and sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody. They are typically produced by immunizing the host with pooled immunoglobulins from the target species and further purified or modified (e.g., immunoaffinity chromatography, fragmentation, conjugation) for high specificity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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