Thermo Fisher Scientific Mouse IgM Isotype Control (11E10), FITC, eBioscience
FITC로 표지된 Mouse IgM 동형 대조 항체로, 면역세포 분석 및 형광 세포 이미징에 적합합니다. Flow cytometry, IHC, ICC 등 다양한 응용에 사용 가능하며, 488 nm 레이저에서 효율적으로 여기됩니다. 4°C 암조건 보관, 동결 금지.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (IHC) | Assay-Dependent |
| Immunocytochemistry (ICC/IF) | Assay-Dependent |
| Flow Cytometry (Flow) | Assay-Dependent |
| Control (Ctrl) | Assay-Dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Host / Isotype | Mouse / IgM |
| Class | Monoclonal |
| Type | Isotype Control |
| Clone | 11E10 |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10547648 |
Additional Formats Available
- Unconjugated
- APC
- eFluor 450
- eFluor 660
- PE-Cyanine7
- Custom conjugation available upon request
Product Specific Information
The mouse IgM is useful as an isotype control immunoglobulin. It was generated by immunization with lipopolysaccharide (LPS) and is reactive with LPS.
This isotype control has been reported for use in immunocytochemistry, immunohistochemistry, and flow cytometric analysis.
It has been pre-titrated and tested by flow cytometric analysis of human peripheral blood leukocytes and can be used at the same concentration as the experimental antibody.
- Excitation: 488 nm
- Emission: 520 nm
- Laser: Blue Laser
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
Mouse IgM is an immunoglobulin M antibody produced by B cells in response to antigens. It is the first antibody produced during an immune response and is the largest immunoglobulin in serum.
Composed of five heavy and five light chains, it plays a crucial role in complement activation and antigen agglutination.
Mouse IgM is widely used in research and diagnostic applications, including ELISA, Western blotting, and immunohistochemistry.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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