Thermo Fisher Scientific DR4 Monoclonal Antibody (DR-4-02)

Applications and Tested Dilution

Product Specifications

Available Formats:

• APC
• FITC
• Request custom conjugation

Product Specific Information

This antibody recognizes an extracellular epitope of TRAIL-R1 (DR4), a human death receptor 4 expressed in most human tissues (spleen, peripheral blood leucocytes, thymus) and in a variety of tumor-derived cell lines.

Endotoxin level is less than 0.01 EU/µg of protein, as determined by the LAL test.

Functional Application:
For blocking, recommended dilution of antibody: 2–3 µg/mL in cultivation medium.
Final concentration of TRAIL: 20–200 ng/mL.
It is recommended to add the antibody 15 min before addition of TRAIL.

Target Information

TRAIL-R1 (CD261, DR4) is a type I transmembrane protein, also called TRAIL receptor 1. The ligand for this DR4 death receptor is TRAIL, a member of the TNF family.
DR4, like other receptors (Fas, TNFR1, etc.), mediates apoptosis and NF-κB activation in many cells and tissues.
Apoptosis, a programmed cell death, is a key process in normal cellular differentiation and development of multicellular organisms. It is induced by cytokines of the TNF family (e.g., TNF, Fas ligand) binding to their death domain-containing receptors (e.g., TNFR1, Fas receptor).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.