Thermo Fisher Scientific DR4 Monoclonal Antibody (DR-4-02)

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC/IF)

Assay-dependent

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Flow Cytometry (Flow)

2-4 µg/mL

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Immunoprecipitation (IP)

Assay-dependent

-

Functional Assay (Functional)

2-3 µg/mL

-

Miscellaneous PubMed (Misc)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

DR-4-02

Immunogen

Fusion protein containing the extracellular part of TRAIL-R1 and the constant part of the heavy chain of the human IgG1.

Conjugate

Unconjugated Unconjugated Unconjugated

View additional formats

• APC
• FITC
• Request custom conjugation

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

no preservative

Storage conditions

4° C, do not freeze

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1088003

Product Specific Information

This antibody recognizes an extracellular epitope of TRAIL-R1 (DR4), a human death receptor 4 expressed in most human tissues (spleen, peripheral blood leucocytes, thymus) and in a variety of tumor-derived cell lines.

Endotoxin level is less than 0.01 EU/ug of the protein, as determined by the LAL test.

Functional Application: For blocking, recommended dilution of antibody: 2-3 µg/mL in cultivation medium, final concentration of TRAIL: 20-200 ng/mL; it is recommended to add the antibody 15 min before addition of TRAIL.

Target Information

TRAIL-R1 (CD261, DR4) is a type I transmembrane protein, also called TRAIL receptor 1. The ligand for this DR4 death receptor has been identified and termed TRAIL, which is a member of the TNF family. DR4, as many other receptors (Fas, TNFR1, etc.), mediates apoptosis and NF kappaB activation in many cells and tissues. Apoptosis, a programmed cell death, is a operating process in normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by coupled of certain cytokines (TNF family - TNF, Fas ligand) and their death domain containing receptors (TNFR1, Fas receptor).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.