Thermo Fisher Scientific SNAP23 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

View 4 publications 4 publications

Immunocytochemistry (ICC/IF)

2 µg/mL

-

Immunoprecipitation (IP)

Assay-dependent

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Bovine, Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Sythetic Peptide: C N(193) K N R I D I A N T R A K K L I D S(210) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:SNAP23,uniProtId:O09044-1,ncbiNodeId:10090,antigenRange:193-210,antigenLength:210,antigenImageFileName:PA1-738_SNAP23_O09044-1_Rabbit.svg,antigenImageFileNamePDP:PA1-738_SNAP23_O09044-1_Rabbit_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2192025

Product Specific Information

PA1-738 detects synaptosomal-associated protein 23 (SNAP-23) from human, mouse and rat samples.

PA1-738 has been successfully used in Western blot and immunoprecipitation procedures. By Western blot, this antibody detects an ~23 kDa protein representing SNAP-23 from rat/mouse brain protein extract and HeLa cell lysates.

PA1-738 immunizing peptide corresponds to amino acid residues 193-210 from mouse SNAP-23 protein. PA1-738 immunizing peptide (Cat. # PEP-147) is available for use in neutralization and control experiments.

Target Information

Specificity of vesicular transport is regulated, in part, by the interaction of a vesicle-associated membrane protein termed synaptobrevin/VAMP with a target compartment membrane protein termed syntaxin. These proteins, together with SNAP25 (synaptosome-associated protein of 25 kDa), form a complex which serves as a binding site for the general membrane fusion machinery. Synaptobrevin/VAMP and syntaxin are believed to be involved in vesicular transport in most, if not all cells, while SNAP25 is present almost exclusively in the brain, suggesting that a ubiquitously expressed homolog of SNAP25 exists to facilitate transport vesicle/target membrane fusion in other tissues. The protein encoded by this gene is structurally and functionally similar to SNAP25 and binds tightly to multiple syntaxins and synaptobrevins/VAMPs. It is an essential component of the high affinity receptor for the general membrane fusion machinery and is an important regulator of transport vesicle docking and fusion. Two alternative transcript variants encoding different protein isoforms have been described for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.